IGEM:MIT/2006/Notebook/2006-6-7: Difference between revisions
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*Toxicity | *Toxicity | ||
*What ranges of concentrations should we be adding | *What ranges of concentrations should we be adding | ||
Banana- 10 | Banana- 10 mM isoamyl alcohol | ||
Jasmine- 1 mM JA and 1 mM S-adenosyl methionine (SAM) | Jasmine- 1 mM JA and 1 mM S-adenosyl methionine (SAM) | ||
Revision as of 15:20, 7 June 2006
Precursor Database
- Transport
- SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
- Biosynthesis (salicylic acid/benzoic acid):
- Biosynthesis (jasmonic acid)
- Many pathways described at http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1066929&blobtype=pdf
- Toxicity
- What ranges of concentrations should we be adding
Banana- 10 mM isoamyl alcohol
Jasmine- 1 mM JA and 1 mM S-adenosyl methionine (SAM)
SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)
Product Database
Sequence analysis
- Restriction sites
- Codon optimization
- Registry accounts
- Install DNA 2.0 software?
Systems & Applications
Things to get
- Precursor molecules etc.
- BL21(DE3)+pLysS competent cells (the Endy Lab has these)
- Arab. JMT
Parts in the registry
<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart> <bbpart>Bba_J45010</bbpart>
