IGEM:MIT/2006/Notebook/2006-6-7

Compound Database

Transport

• SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
• BA was added exogenously, and methyl benzoate (MB) was produced, so BA can enter E. coli. MB concentrations were measured without needing to lyse the cells, so MB can exit the cell. (Reference; it is the first paper under this section)

Biosynthesis

salicylic acid/benzoic acid

http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif

jasmonic acid

• Many pathways described at http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=1066929&blobtype=pdf

Toxicity

So I just found this study where they looked at BA-induced overexpression of proteins in E. coli. I am going through it right now. Here is the reference, though:

1. Lambert LA, Abshire K, Blankenhorn D, and Slonczewski JL. Proteins induced in Escherichia coli by benzoic acid. J Bacteriol. 1997 Dec;179(23):7595-9. DOI:10.1128/jb.179.23.7595-7599.1997 |

Concentration

What ranges of concentrations should we be adding?

banana

• 10 mM isoamyl alcohol

jasmine

• 1 mM JA and 1 mM S-adenosyl methionine (SAM)

salicylic acid or benzoic acid

• SAMT (A. Majus and C. breweri) 5 μg/ml of SA (or BA)

Sequence analysis

• Restriction sites
• Codon optimization
• Registry accounts
• Install DNA 2.0 software?

Things to get

• Precursor molecules etc.
• BL21(DE3)+pLysS competent cells (the Endy Lab has these)
• Arab. JMT

Parts in the registry

<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart> <bbpart>Bba_J45010</bbpart>