IGEM:MIT/2006/Notebook/2006-6-7

Precursor Database

• Transport
  • SA can be transported into/out of tobacco cells (http://ejournal.sinica.edu.tw/bbas/content/1999/4/bot404-03.html)
• Biosynthesis (salicylic acid/benzoic acid):
  • http://www.hort.purdue.edu/rhodcv/hort640c/secprod/sec7.gif"
• Toxicity
• What ranges of concentrations should we be adding

Banana- 10-15 mM isoamyl alcohol Jasmine- 1 mM JA and 1 mM S-adenosyl methionine (SAM)

Sequence analysis

• Restriction sites
• Codon optimization
• Registry accounts
• Install DNA 2.0 software?

Things to get

• Precursor molecules etc.
• BL21(DE3)+pLysS competent cells (the Endy Lab has these)
• Arab. JMT

Parts in the registry

<bbpart>BBa_J45000</bbpart> <bbpart>BBa_J45001</bbpart> <bbpart>BBa_J45002</bbpart> <bbpart>BBa_J45003</bbpart> <bbpart>BBa_J45004</bbpart> <bbpart>BBa_J45005</bbpart> <bbpart>BBa_J45006</bbpart> <bbpart>BBa_J45007</bbpart> <bbpart>Bba_J45010</bbpart>