IGEM:MIT/2006/Notebook/2006-6-8

SAMT from A. Majus

1. Forward Strand
   • Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
   • Length of matching: 21 bp
   • GC content: 28.6%
   • Melt Temp. 48.0 C

1. Reverse Strand
   • Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC -3'
   • Length of matching: 21 bp
   • GC content: 38.1.0%
   • Melt Temp. 50.4 C

BAMT from A. majus

• Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
  • Length: 47
  • GC Content: 31.6%
  • Melting Temp: 45.2

• Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
  • Length: 53
  • GC Content: 38.9%
  • Melting Temp: 44.3

BSMT from P. x hybrida

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'

• LENGTH: 49
  
• GC CONTENT: 46.9 %
  
• MELT TEMP: 67.6 ºC
  

Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAC -3'

• LENGTH: 51
  
• GC CONTENT: 39.2 %
  
• MELT TEMP: 65.0 ºC
  

Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA C -3'

• LENGTH: 52
  
• GC CONTENT: 38.5 %
  
• MELT TEMP: 65.0 ºC
  

ATF1 from S. Cerevisiae

Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'

• Length: 46 bp
• GC content: 45.7%
• Melt temp: 66.4 deg. C

Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'

• Length: 52 bp
• GC content: 48.1%
• Melt temp: 67.9 deg. C