IGEM:MIT/2006/Notebook/2006-6-8: Difference between revisions
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<br> | <br> | ||
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT T<B>AA TTT ATT TTG GTC AAA GAG AC</b> -3'<br> | Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT T<B>AA TTT ATT TTG GTC AAA GAG AC</b> -3'<br> | ||
*LENGTH: | *LENGTH: 22 <br> | ||
*GC CONTENT: | *GC CONTENT: 27.3 % <br> | ||
*MELT TEMP: | *MELT TEMP: 47.1 ºC <br> | ||
==ATF1 from ''S. Cerevisiae''== | ==ATF1 from ''S. Cerevisiae''== |
Revision as of 08:44, 9 June 2006
PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG C -3'
- Length of matching: 22 bp
- GC content: 31.8%
- Melt Temp. 51.0 C
- Reverse Strand
- Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC -3'
- Length of matching: 21 bp
- GC content: 38.1.0%
- Melt Temp. 50.4 C
BAMT from A. majus
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
- Length: 47
- GC Content: 31.6%
- Melting Temp: 45.2
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
- Length: 53
- GC Content: 38.9%
- Melting Temp: 44.3
BSMT from P. x hybrida
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA GAG AC -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 47.1 ºC
ATF1 from S. Cerevisiae
Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'
- Length: 46 bp
- GC content: 45.7%
- Melt temp: 66.4 deg. C
- Length only anneal: 18
- GC content only anneal: 33.3%
- Melt temp only anneal: 44.2 deg. C
Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'
- Length: 52 bp
- GC content: 48.1%
- Melt temp: 67.9 deg. C
- Length only anneal: 23
- GC content only anneal: 39.1%
- Melt temp only anneal: 51.9 deg. C