IGEM:MIT/2006/Notebook/2006-6-8

SAMT from A. Majus

1. Forward Strand
   • Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG C -3'
   • Length of matching: 22 bp
   • GC content: 31.8%
   • Melt Temp. 51.0 C
   • Whole sequence analysis
     • Lowest secondary structure deltaG: -.9 kcal/mol
     • No significant homodimers or heterodimers

1. Reverse Strand
   • Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC -3'
   • Length of matching: 21 bp
   • GC content: 38.1.0%
   • Melt Temp. 50.4 C
   • Whole sequence analysis
     • Lowest secondary structure deltaG: -1.68 kcal/mol
     • No significant homodimers or heterodimers

BAMT from A. majus

• Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
  • Length: 47
  • GC Content: 31.6%
  • Melting Temp: 45.2

• Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
  • Length: 53
  • GC Content: 38.9%
  • Melting Temp: 44.3

BSMT from P. x hybrida

Designed Forward Strand (1 and 2): 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT CTT C -3'

• LENGTH: 22
  
• GC CONTENT: 36.4 %
  
• MELT TEMP: 51.1 ºC
  

Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'

• LENGTH: 22
  
• GC CONTENT: 27.3 %
  
• MELT TEMP: 46.6 ºC
  

Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA GAG AC -3'

• LENGTH: 22
  
• GC CONTENT: 27.3 %
  
• MELT TEMP: 47.1 ºC
  

ATF1 from S. Cerevisiae

Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'

• Length: 46 bp
• GC content: 45.7%
• Melt temp: 66.4 deg. C
• Length only anneal: 18
• GC content only anneal: 33.3%
• Melt temp only anneal: 44.2 deg. C

Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'

• Length: 52 bp
• GC content: 48.1%
• Melt temp: 67.9 deg. C
• Length only anneal: 23
• GC content only anneal: 39.1%
• Melt temp only anneal: 51.9 deg. C