IGEM:MIT/2006/Notebook/2006-6-8: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 27: | Line 27: | ||
**Melting Temp: 45.2 | **Melting Temp: 45.2 | ||
*Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ''TTA TTA'' '''TCT CCT ACT TAG AGA AAC''' | *Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ''TTA TTA'' '''TCT CCT ACT TAG AGA AAC TAC''' | ||
**Length: 53 | **Length: 53 | ||
**GC Content: 38.9% | **GC Content: 38.9% | ||
Revision as of 08:56, 9 June 2006
PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG C
- Length of matching: 22 bp
- GC content: 31.8%
- Melt Temp. 51.0 C
- Whole sequence analysis
- Lowest secondary structure deltaG: -.9 kcal/mol
- No significant homodimers or heterodimers
- Reverse Strand
- Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC
- Length of matching: 21 bp
- GC content: 38.1.0%
- Melt Temp. 50.4 C
- Whole sequence analysis
- Lowest secondary structure deltaG: -1.68 kcal/mol
- No significant homodimers or heterodimers
BAMT from A. majus
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
- Length: 47
- GC Content: 31.6%
- Melting Temp: 45.2
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC TAC
- Length: 53
- GC Content: 38.9%
- Melting Temp: 44.3
BSMT from P. x hybrida
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 19
- GC CONTENT: 36.8 %
- MELT TEMP: 47.8 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAG GAG -3'
- LENGTH: 22
- GC CONTENT: 27.3 %
- MELT TEMP: 46.6 ºC
ATF1 from S. Cerevisiae
Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'
- Length: 46 bp
- GC content: 45.7%
- Melt temp: 66.4 deg. C
- Length only anneal: 18
- GC content only anneal: 33.3%
- Melt temp only anneal: 44.2 deg. C
- DeltaG hairpin: -1.52 kcal/mol
- DeltaG homodimer: -91.97 kcal/mol
Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'
- Length: 52 bp
- GC content: 48.1%
- Melt temp: 67.9 deg. C
- Length only anneal: 23
- GC content only anneal: 39.1%
- Melt temp only anneal: 51.9 deg. C
- DeltaG hairpin: -2.98 kcal/mol
- DeltaG homodimer: -102.44 kcal/mol
DeltaG heterodimer: -102.44 kcal/mol
