IGEM:MIT/2006/Notebook/2006-6-8: Difference between revisions
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==BSMT (1 and 2) from ''P. x hybrida''== | ==BSMT (1 and 2) from ''P. x hybrida''== | ||
Designed Forward | Designed Forward Strand: <Br> | ||
GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT] <br> | 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT] -3'<br> | ||
LENGTH: 48<br> | LENGTH: 48<br> | ||
GC CONTENT: 45.8 %<br> | GC CONTENT: 45.8 %<br> | ||
MELT TEMP: 67.7 ºC<br> | MELT TEMP: 67.7 ºC<br> | ||
<br> | <br> | ||
Designed Reverse | Designed Reverse Strand: | ||
GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA]<br> | 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA] -3'<br> | ||
LENGTH: 50<br> | LENGTH: 50<br> | ||
GC CONTENT: 38.0 %<br> | GC CONTENT: 38.0 %<br> | ||
MELT TEMP: 64.9 ºC<br> | MELT TEMP: 64.9 ºC<br> | ||
Revision as of 14:17, 8 June 2006
PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
- Length: 51 bp
- GC content: 43.1%
- Melt Temp. 67.0 C
BAMT from A. majus
BSMT (1 and 2) from P. x hybrida
Designed Forward Strand:
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG [ATG GAA GTT GTT GAA GTT] -3'
LENGTH: 48
GC CONTENT: 45.8 %
MELT TEMP: 67.7 ºC
Designed Reverse Strand:
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA[T TAT TAA TTT ATT TTG GTC AA] -3'
LENGTH: 50
GC CONTENT: 38.0 %
MELT TEMP: 64.9 ºC
