IGEM:MIT/2006/Notebook/2006-6-8: Difference between revisions
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==BSMT (1 and 2) from ''P. x hybrida''== | ==BSMT (1 and 2) from ''P. x hybrida''== | ||
Designed Forward Strand: | Designed Forward Strand: | ||
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG | 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG <b>ATG GAA GTT GTT GAA GTT</b> -3'<br> | ||
LENGTH: 48<br> | LENGTH: 48<br> | ||
GC CONTENT: 45.8 %<br> | GC CONTENT: 45.8 %<br> | ||
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<br> | <br> | ||
Designed Reverse Strand: | Designed Reverse Strand: | ||
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA | 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TA<b>T TAT TAA TTT ATT TTG GTC AA</b> -3'<br> | ||
LENGTH: 50<br> | LENGTH: 50<br> | ||
GC CONTENT: 38.0 %<br> | GC CONTENT: 38.0 %<br> | ||
MELT TEMP: 64.9 ºC<br> | MELT TEMP: 64.9 ºC<br> | ||
Revision as of 14:22, 8 June 2006
PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
- Length: 51 bp
- GC content: 43.1%
- Melt Temp. 67.0 C
- Reverse Strand
- Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAG TTT CTT TTT GCT TG -3'
- Length: 50 bp
- GC content: 42.0%
- Melt Temp. 65.9 C
BAMT from A. majus
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG AAA GTG ATG AAG AAA C
- Specs:
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
- Specs:
BSMT (1 and 2) from P. x hybrida
Designed Forward Strand:
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT -3'
LENGTH: 48
GC CONTENT: 45.8 %
MELT TEMP: 67.7 ºC
Designed Reverse Strand:
5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AA -3'
LENGTH: 50
GC CONTENT: 38.0 %
MELT TEMP: 64.9 ºC
