IGEM:MIT/2006/Notebook/2006-6-8: Difference between revisions
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*Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG AAA GTG ATG AAG AAA C''' | *Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G '''ATG AAA GTG ATG AAG AAA C''' | ||
**Length: | **Length: 47 | ||
**GC Content: | **GC Content: 31.6% | ||
**Melting Temp: | **Melting Temp: 45.2 | ||
*Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ''TTA TTA'' '''TCT CCT ACT TAG AGA AAC''' | *Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA ''TTA TTA'' '''TCT CCT ACT TAG AGA AAC''' | ||
**Length: 53 | **Length: 53 | ||
**GC Content: | **GC Content: 38.9% | ||
**Melting Temp: | **Melting Temp: 44.3 | ||
==BSMT from ''P. x hybrida''== | ==BSMT from ''P. x hybrida''== |
Revision as of 08:29, 9 June 2006
PRIMER INFORMATION FOR ORDERING
SAMT from A. Majus
- Forward Strand
- Sequence to order: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG ACA AAA CAA ACA CAA AAG -3'
- Length: 51 bp
- GC content: 43.1%
- Melt Temp. 67.0 C
- Reverse Strand
- Sequence to order: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TA GTT TCT TTT TGC TTG TCA CTC -3'
- Length: 50 bp
- GC content: 42.0%
- Melt Temp. 65.9 C
BAMT from A. majus
- Forward primer (5' -> 3'): GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAA GTG ATG AAG AAA C
- Length: 47
- GC Content: 31.6%
- Melting Temp: 45.2
- Reverse primer (5' -> 3'): GTT TCT TCC TGC AGC GGC CGC TAC TAG TA TTA TTA TCT CCT ACT TAG AGA AAC
- Length: 53
- GC Content: 38.9%
- Melting Temp: 44.3
BSMT from P. x hybrida
Designed Forward Strand (1 and 2):
5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA GAG ATG GAA GTT GTT GAA GTT C -3'
- LENGTH: 49
- GC CONTENT: 46.9 %
- MELT TEMP: 67.6 ºC
Designed Reverse Strand (phBSMT1): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAC -3'
- LENGTH: 51
- GC CONTENT: 39.2 %
- MELT TEMP:
Designed Reverse Strand (phBSMT2): 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA TTT ATT TTG GTC AAA C -3'
- LENGTH: 52
- GC CONTENT: 38.5 %
- MELT TEMP: 65.0 ºC
ATF1 from S. Cerevisiae
Designed forward primer: 5'- GTT TCT TCG AAT TCG CGG CCG CTT CTA G ATG AAT GAA ATC GAT GAG -3'
- Length: 48 bp
- GC content: 45.8%
- Melt temp: 66.7 deg. C
Designed reverse primer: 5'- GTT TCT TCC TGC AGC GGC CGC TAC TAG TAT TAT TAA GGG CCT AAA AGG AGA G -3'
- Length: 52 bp
- GC content: 48.1%
- Melt temp: 67.9 deg. C