IGEM:MIT/2006/Notebook/2006-6-9: Difference between revisions
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#Add approriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT). | #Add approriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT). | ||
#Carefully cut out paper that contains DNA and place into Eppen. | #Carefully cut out paper that contains DNA and place into Eppen. | ||
#*Ensure | #*Ensure submergence with toothpick | ||
#Followed transformation protocol detailed: http://openwetware.org/wiki/Transforming_chemically_competent_cells | #Followed transformation protocol detailed: http://openwetware.org/wiki/Transforming_chemically_competent_cells | ||
#*Used 2μL of plasmid/DNA. | #*Used 2μL of plasmid/DNA. | ||
Revision as of 12:23, 9 June 2006
Here's a page explaining why the BioBricks prefix for coding regions is different from the BioBricks prefix for all other parts as described by Reshma this morning.
Protocols
Dehydrated DNA Recovery (from paper)
- Final DNA Concentration: 1ng/μL
- Add approriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
- Carefully cut out paper that contains DNA and place into Eppen.
- Ensure submergence with toothpick
- Followed transformation protocol detailed: http://openwetware.org/wiki/Transforming_chemically_competent_cells
- Used 2μL of plasmid/DNA.
