IGEM:MIT/2006/Notebook/2006-6-9: Difference between revisions
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[http://parts.mit.edu/r/parts/htdocs/Assembly/rbs_cds.cgi Here's] a page explaining why the BioBricks prefix for coding regions is different from the BioBricks prefix for all other parts as described by Reshma this morning. | [http://parts.mit.edu/r/parts/htdocs/Assembly/rbs_cds.cgi Here's] a page explaining why the BioBricks prefix for coding regions is different from the BioBricks prefix for all other parts as described by Reshma this morning. | ||
== | ==06/09/06 Preliminary Transformation Lab== | ||
===Dehydrated DNA Recovery (from paper)=== | ===Dehydrated DNA Recovery (from paper)=== | ||
Revision as of 12:24, 9 June 2006
Here's a page explaining why the BioBricks prefix for coding regions is different from the BioBricks prefix for all other parts as described by Reshma this morning.
06/09/06 Preliminary Transformation Lab
Dehydrated DNA Recovery (from paper)
- Final DNA Concentration: 1ng/μL
- Add approriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
- Carefully cut out paper that contains DNA and place into Eppen.
- Ensure submergence with toothpick
- Wait for 10 minutes
- Followed transformation protocol detailed: http://openwetware.org/wiki/Transforming_chemically_competent_cells
- Used 2μL of plasmid/DNA.
