IGEM:MIT/2006/Notebook/2006-6-9: Difference between revisions

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===Sources===
===Sources===
*Note, used 1/2 of designated source
#SAMT in PET28 1.6 μg
#SAMT in PET28 1.6 μg
#BAMT in PET28 2.0 μg
#BAMT in PET28 2.0 μg
Line 11: Line 12:
*Final DNA Concentration: 1ng/μL
*Final DNA Concentration: 1ng/μL


#Add approriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
#Add appropriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
#Carefully cut out paper that contains DNA and place into Eppen.
#Carefully cut out paper that contains DNA and place into Eppen.
#*Ensure submergence with toothpick  
#*Ensure submergence with toothpick  

Revision as of 12:28, 9 June 2006

Here's a page explaining why the BioBricks prefix for coding regions is different from the BioBricks prefix for all other parts as described by Reshma this morning.

06/09/06 Preliminary Transformation Lab

Sources

  • Note, used 1/2 of designated source
  1. SAMT in PET28 1.6 μg
  2. BAMT in PET28 2.0 μg
  3. BSMT in PET28 2.0 μg

Dehydrated DNA Recovery (from paper)

  • Final DNA Concentration: 1ng/μL
  1. Add appropriate amount of TE buffer to 1.7mL Eppendorf tubes (500μL for BAMT and BSMT; 400μL for SAMT).
  2. Carefully cut out paper that contains DNA and place into Eppen.
    • Ensure submergence with toothpick
    • Wait for 10 minutes

Transformation

  1. Followed transformation protocol detailed: http://openwetware.org/wiki/Transforming_chemically_competent_cells
    • Used 2μL of plasmid/DNA.
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