IGEM:MIT/2006/Notebook/2006-7-10: Difference between revisions

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*We already have the necessary primers (VR1 and VF2).
*We already have the necessary primers (VR1 and VF2).
*'''Note''': You only set up the sequencing reaction with '''one''' of these two primers
*'''Note''': You only set up the sequencing reaction with '''one''' of these two primers
*Add the following volumes for a'''10'''-reaction master-mix:
*Add the following volumes for a '''10'''-reaction master-mix:
**90&mu;L H<sub>2</sub>O
**90&mu;L H<sub>2</sub>O
**1&mu;L primer
**1&mu;L primer
*Add 9.01&mu;L to each tube
*Add 9.01&mu;L to each tube
*Add 3&mu;L DNA
*Add 3&mu;L respective DNA

Revision as of 14:04, 7 July 2006

Sequencing

  • Refer here for sequencing protocol.
  • We already have the necessary primers (VR1 and VF2).
  • Note: You only set up the sequencing reaction with one of these two primers
  • Add the following volumes for a 10-reaction master-mix:
    • 90μL H2O
    • 1μL primer
  • Add 9.01μL to each tube
  • Add 3μL respective DNA
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