IGEM:MIT/2006/Notebook/2006-7-10: Difference between revisions
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*Add 9.01μL to each tube | *Add 9.01μL to each tube | ||
*Add 3μL respective DNA | *Add 3μL respective DNA | ||
''' | |||
''Reaction Wells'' | ''Reaction Wells''''' | ||
1. SAMT-A VF2 | 1. SAMT-A VF2 |
Revision as of 07:20, 10 July 2006
Sequencing
- Refer here for sequencing protocol.
- We already have the necessary primers (VR1 and VF2).
- Note: You only set up the sequencing reaction with one of these two primers
- Add the following volumes for a 10-reaction master-mix:
- 90μL H2O
- 1μL primer
- Add 9.01μL to each tube
- Add 3μL respective DNA
Reaction Wells
1. SAMT-A VF2
2. SAMT-B VF2
3. SAMT-C VF2
4. SAMT-D VF2
5. BSMT-A VF2
6. BSMT-C VF2
7. BSMT-E VF2
8. BAMT VF2
9. SAMT-A VR
10. SAMT-B VR
11. SAMT-C VR
12. SAMT-D VR
13. BSMT-A VR
14. BSMT-C VR
15. BSMT-E VR
16. BAMT VR
17. ATF1 VF2
18. ATF1 VR