IGEM:MIT/2006/Notebook/2006-7-18: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 19: | Line 19: | ||
5' | 5'-ctagaggcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaaatactagtagcggccgctgca-3' | ||
Length: 84 bases | |||
GC Content: 41.7% | |||
Melting Temperature: 69.7 degrees Celsius | |||
Complementary Strand: | Complementary Strand: | ||
| Line 25: | Line 31: | ||
5'-GCGGCCGCTACTAGTATTTGTTAAATATAGATCACAATTTTGAAACCGCTCGGGATATCAGCGAAAACATAAGCCT-3' | 5'-GCGGCCGCTACTAGTATTTGTTAAATATAGATCACAATTTTGAAACCGCTCGGGATATCAGCGAAAACATAAGCCT-3' | ||
Length: 76 bases | |||
GC Content: 40.8% | |||
Melting Temperature: 69.1 degrees Celsius | |||
Heterodimer DeltaG: -161.93 kcal/mol | |||
Also, I contacted Professor Schellhorn last night inquiring about a plasmid containing osmY or the sequences of the primers he used to PCR osmY out of E. coli. | Also, I contacted Professor Schellhorn last night inquiring about a plasmid containing osmY or the sequences of the primers he used to PCR osmY out of E. coli. | ||
Revision as of 11:22, 18 July 2006
Transformants
- IK control did not grow, though BSMT and BAMT grew
- ATF1 and Top10 control grew as well
- Next step is liquid culture all of them, and miniprep on ATF1
- SAMTC connected to both RBSs grew along with SAMTA connected to both RBSs and the control.
Meeting
- Tomorrow: Sequence: 4 SAMT-C connected to rbs & term-30, and 4 SAMT-C connected to rbs & term-32
- end of the day: Liquid culture all transformants (Duda. BAMT in indole-knockout, Duda. BSMT in indole-knockout, the two above)
- Make liquid culture from the glycerols of the constitutive promoters
- Veena: miniprep pCHBA, sequence it with primers from Reshma
- Andre- request plasmid for vanilla
- maybe: re-cut miniprepped BSMT
- research exponential phase promoter
Annealing Parts for Synthesis of the osmY Promoter
Forward Strand:
5'-ctagaggcttatgttttcgctgatatcccgagcggtttcaaaattgtgatctatatttaacaaatactagtagcggccgctgca-3'
Length: 84 bases
GC Content: 41.7%
Melting Temperature: 69.7 degrees Celsius
Complementary Strand:
5'-GCGGCCGCTACTAGTATTTGTTAAATATAGATCACAATTTTGAAACCGCTCGGGATATCAGCGAAAACATAAGCCT-3'
Length: 76 bases
GC Content: 40.8%
Melting Temperature: 69.1 degrees Celsius
Heterodimer DeltaG: -161.93 kcal/mol
Also, I contacted Professor Schellhorn last night inquiring about a plasmid containing osmY or the sequences of the primers he used to PCR osmY out of E. coli.
