IGEM:MIT/2006/Notebook/2006-7-19

Smell Cultures

1. 4 BSMT (2 SA, 2 BA) and 2 BAMT (ctrl not induced)

Sequencing Results

Positive for SAMT.B0015 C! The one we used!

Digests

R0040

27.5 uL H20

5 uL Buffer 2

.5 uL BSA

16 uL 50 ng/uL DNA

.5 uL EcoRI

.5 uL SpeI

B0030.SAMT.B0015

27.5 uL H20

5 uL Buffer 2

.5 uL BSA

16 uL 50 ng/uL DNA

.5 uL EcoRI

.5 uL XbaI

B0030.SAMT.B0015 A, B, & C + B0032.SAMT.B0015 A, B, C, & D

35.5 uL H20

5 uL Buffer 2

.5 uL BSA

8 uL 100 ng/uL DNA

.5 uL EcoRI

.5 uL XbaI

ATF1 Mutagenesis A & B

36 uL H20

5 uL Buffer 2

.5 uL BSA

8 uL 100 ng/uL DNA

.5 uL EcoRI