IGEM:MIT/2006/Notebook/2006-7-20: Difference between revisions
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==To do today== | |||
*make other indole knockout strains competent | |||
*re-do the smell experiment with indole-knockout strain that we have already cultured | |||
*primers for SA-generator | |||
*liquid cultures of the transformants (promoter-RBS-SAMT-terminator) | |||
*if BAMT mutagenesis worked, transform BAMT into BL21 ''find competent BL21s!!'' | |||
==Transfomants== | ==Transfomants== | ||
Revision as of 07:37, 20 July 2006
To do today
- make other indole knockout strains competent
- re-do the smell experiment with indole-knockout strain that we have already cultured
- primers for SA-generator
- liquid cultures of the transformants (promoter-RBS-SAMT-terminator)
- if BAMT mutagenesis worked, transform BAMT into BL21 find competent BL21s!!
Transfomants
Transformants were seen on every plate but the R0040.B0030.SAMT.B0015 D plate!!! We hopefully have the wintergreen generating device completed!!!
Meeting: 1:30 PM
We'll talk about our system diagram:
