IGEM:MIT/2006/Notebook/2006-7-20: Difference between revisions
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==To do today== | ==To do today== | ||
*make other indole knockout strains competent | *make other indole knockout strains competent | ||
*re-do the smell experiment with indole-knockout strain that we have already cultured | *re-do the smell experiment with indole-knockout strain that we have already cultured (*edit not necessary- need entire construct) | ||
*primers for SA-generator | *primers for SA-generator | ||
*liquid cultures of the transformants (promoter-RBS-SAMT-terminator) | *liquid cultures of the transformants (promoter-RBS-SAMT-terminator) | ||
Revision as of 12:22, 20 July 2006
To do today
- make other indole knockout strains competent
- re-do the smell experiment with indole-knockout strain that we have already cultured (*edit not necessary- need entire construct)
- primers for SA-generator
- liquid cultures of the transformants (promoter-RBS-SAMT-terminator)
- if BAMT mutagenesis worked, transform BAMT into BL21 find competent BL21s!!
Transfomants
Transformants were seen on every plate but the R0040.B0030.SAMT.B0015 D plate!!! We hopefully have the wintergreen generating device completed!!!
Meeting: 2:00 PM
We'll talk about our system diagram:
