IGEM:MIT/2006/Notebook/2006-7-20

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To do today

  • make other indole knockout strains competent
  • re-do the smell experiment with indole-knockout strain that we have already cultured (*edit not necessary- need entire construct)
  • primers for SA-generator
  • liquid cultures of the transformants (promoter-RBS-SAMT-terminator)
  • if BAMT mutagenesis worked, transform BAMT into BL21 find competent BL21s!!

Transfomants

Transformants were seen on every plate but the R0040.B0030.SAMT.B0015 D plate!!! We hopefully have the wintergreen generating device completed!!!

Meeting: 2:00 PM

We'll talk about our system diagram: