IGEM:MIT/2006/Notebook/2006-7-24: Difference between revisions
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Done with each pair of new primers meant for PCRing both the short and long osmY promoter region. | Done with each pair of new primers meant for PCRing both the short and long osmY promoter region. | ||
45μL PCR SuperMix | *45μL PCR SuperMix | ||
2.5μL 0.55ng/μL E. coli genome | *2.5μL 0.55ng/μL E. coli genome | ||
1.25μL each respective primer (25 ng/μL) | *1.25μL each respective primer (25 ng/μL) | ||
Revision as of 08:28, 24 July 2006
Smell Experiments w/ Isoamyl Alcohol
- Questions that we want to answer:
- Does IAA smell like banana? Yes
- Does IAA smell strongly in LB?
- At what IAA concentration can E. coli "normal" growth?
- Culture Top10s and add different concentrations of IAA after cells reach OD ~ 0.5
osmY PCR
Done with each pair of new primers meant for PCRing both the short and long osmY promoter region.
- 45μL PCR SuperMix
- 2.5μL 0.55ng/μL E. coli genome
- 1.25μL each respective primer (25 ng/μL)
