IGEM:MIT/2006/Notebook/2006-7-24

Smell Experiments w/ Isoamyl Alcohol

• Questions that we want to answer:
  1. Does IAA smell like banana? Yes
  2. Does IAA smell strongly in LB?
  3. At what IAA concentration can E. coli "normal" growth?
     • Culture Top10s and add different concentrations of IAA after cells reach OD ~ 0.5

osmY PCR

Done with each pair of new primers meant for PCRing both the short and long osmY promoter region.