IGEM:MIT/2006/Notebook/2006-7-26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 15: | Line 15: | ||
==Digest ATF1 for hooking up to B0030(2)== | ==Digest ATF1 for hooking up to B0030(2)== | ||
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2) | ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2) | ||
==Cut B0015 with EX== | |||
Revision as of 08:01, 26 July 2006
SPP Digest
Digested the annealed SPP again- last time used wrong spin column to cleanup ->don't need to do this. the annealed SPP is already cut with E and P
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
