IGEM:MIT/2006/Notebook/2006-7-26: Difference between revisions
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==Cut backbone with EP== | ==Cut backbone with EP== | ||
EP backbone for inserting the SPP | EP backbone for inserting the SPP | ||
==Liquid Cultures== | |||
End of the day liquid cultures for future use: ATF1, R0040, B0030(2) | |||
Revision as of 08:29, 26 July 2006
SPP Digest
Digested the annealed SPP again- last time used wrong spin column to cleanup ->don't need to do this. the annealed SPP is already cut with E and P
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP to hook up the two
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
Cut B0015 with EX
Will hook up later
Cut backbone with EP
EP backbone for inserting the SPP
Liquid Cultures
End of the day liquid cultures for future use: ATF1, R0040, B0030(2)
