IGEM:MIT/2006/Notebook/2006-7-26: Difference between revisions
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==Digest B0030(2) and CDS+Terminator== | ==Digest B0030(2) and CDS+Terminator== | ||
Cut B0030(2) with SP and CDS+Term with XP to hook up the two | Cut B0030(2) with SP and CDS+Term with XP to hook up the two | ||
*'''will cut B0030(2) tomorrow b/c ran out of miniprep''' | |||
==Digest ATF1 for hooking up to B0030(2)== | ==Digest ATF1 for hooking up to B0030(2)== | ||
Revision as of 12:11, 26 July 2006
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP to hook up the two
- will cut B0030(2) tomorrow b/c ran out of miniprep
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
Cut B0015 with EX
Will hook up later
Cut backbone with EP
EP backbone for inserting the SPP
LIGATIONS
- promoter R0040 + rbs B0030
- promoter R0040 + rbs B0032
- stationary phase promoter osmY + backbone pSB1A3-1
Liquid Cultures
End of the day liquid cultures for future use: ATF1, R0040, B0030(2)
