IGEM:MIT/2006/Notebook/2006-7-26: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 1: | Line 1: | ||
==Digest R0040 and B0030(2)== | ==Digest R0040 and B0030(2)== | ||
Line 25: | Line 24: | ||
#promoter R0040 + rbs B0032 | #promoter R0040 + rbs B0032 | ||
#stationary phase promoter osmY + backbone pSB1A3-1 | #stationary phase promoter osmY + backbone pSB1A3-1 | ||
==Transformations== | |||
==Liquid Cultures== | ==Liquid Cultures== | ||
End of the day liquid cultures for future use: ATF1, R0040, B0030(2) | End of the day liquid cultures for future use: ATF1, R0040, B0030(2) |
Revision as of 12:12, 26 July 2006
Digest R0040 and B0030(2)
Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS
Miniprep B0015
Did 3 minipreps of the terminator for general use
Digest B0030(2) and CDS+Terminator
Cut B0030(2) with SP and CDS+Term with XP to hook up the two
- will cut B0030(2) tomorrow b/c ran out of miniprep
Digest ATF1 for hooking up to B0030(2)
ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)
Cut B0015 with EX
Will hook up later
Cut backbone with EP
EP backbone for inserting the SPP
LIGATIONS
- promoter R0040 + rbs B0030
- promoter R0040 + rbs B0032
- stationary phase promoter osmY + backbone pSB1A3-1
Transformations
Liquid Cultures
End of the day liquid cultures for future use: ATF1, R0040, B0030(2)