IGEM:MIT/2006/Notebook/2006-7-26

SPP Digest

Digested the annealed SPP again- last time used wrong spin column to cleanup ->don't need to do this. the annealed SPP is already cut with E and P

Digest R0040 and B0030(2)

Digested R0040 with E,S and B0030 with E,X
We want to hook up both the promoter and RBS

Miniprep B0015

Did 3 minipreps of the terminator for general use

Digest B0030(2) and CDS+Terminator

Cut B0030(2) with SP and CDS+Term with XP to hook up the two

Digest ATF1 for hooking up to B0030(2)

ATF1 cut with EX, cut again with P to hook up to to SP cut B0030(2)

Cut B0015 with EX

Will hook up later

Cut backbone with EP

EP backbone for inserting the SPP

Liquid Cultures

End of the day liquid cultures for future use: ATF1, R0040, B0030(2)