IGEM:MIT/2006/Notebook/2006-7-27
From OpenWetWare
To Do
- Miniprep overnight cultures
- Ligations + Digestions
- Pour more LB Amp plates
- Retransform pchBA and osmY promoter + pSB1A3-1
- Transform ligations
Digestions
- E0840 w/ EX
- osmY w/ S
- ATF1-1148 w/ ES
- B0030 w/ SP
- B0032 w/ SP
Ligations
- B0030(2)SP + 45001.B0015CXP
- B0030(2)SP + ATF1-1148XP
- osmYES + E0840EX
- ATF1-1148ES + B0015EX
Transformations (using new cells from Tom)
- B0030(2)SP + 45001.B0015CXP
- B0030(2)SP + ATF1-1148XP
- osmYES + E0840EX
- ATF1-1148ES + B0015EX
- pchBA w/ pchA silent mutation (transformation failed yesterday)
- osmY + pSB1A3 backbone (transformation failed yesterday)
- pUC19
Desired Completed Constructs
- RBS-SAMT-Term
- RBS-ATF1
- SPP-RBS-GFP-Term
- ATF1-Term
Listed Current Biobricks
Can be found at [[../../Index#List of Biobrick Molecules | in the index]]
Made liquid cultures of transformants
- R0040+B0030 [A, B, C, and D] (in LBAmp)
- R0040+B0032 (in LBAmp)