IGEM:MIT/2006/Notebook/2006-7-28: Difference between revisions
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#*1 μL pME3366 template | #*1 μL pME3366 template | ||
#*.6 μL pchB Forward | #*.6 μL pchB Forward | ||
#.6 μL pchA Reverse | #*.6 μL pchA Reverse | ||
#Tube 2 (pchB): | #Tube 2 (pchB): | ||
#*49 μL PCR supermix | #*49 μL PCR supermix | ||
#*1 μL pME3366 template | #*1 μL pME3366 template | ||
#*.6 μL pchB Forward | #*.6 μL pchB Forward | ||
#.6 μL pchB Reverse | #*.6 μL pchB Reverse | ||
#Tube 3 (pchA): | #Tube 3 (pchA): | ||
#*49 μL PCR supermix | #*49 μL PCR supermix | ||
#*1 μL pME3366 template | #*1 μL pME3366 template | ||
#*.6 μL pchA Forward | #*.6 μL pchA Forward | ||
#.6 μL pchA Reverse | #*.6 μL pchA Reverse |
Revision as of 07:49, 28 July 2006
Amazing News
Transformants on every plate!!!
To do
Miniprep 5 liquid cultures
pchBA PCR
- Lets try to amplify out pchBA together and seperately
- 3 test tubes + 1 control
- Dilute new Invitrogen pchBA primers into working stocks
- Tube 1 (pchBA):
- Tube 1 (pchBA):
- 49 μL PCR supermix
- 1 μL pME3366 template
- .6 μL pchB Forward
- .6 μL pchA Reverse
- Tube 2 (pchB):
- 49 μL PCR supermix
- 1 μL pME3366 template
- .6 μL pchB Forward
- .6 μL pchB Reverse
- Tube 3 (pchA):
- 49 μL PCR supermix
- 1 μL pME3366 template
- .6 μL pchA Forward
- .6 μL pchA Reverse