IGEM:MIT/2006/Notebook/2006-7-28

Amazing News

Transformants on every plate!!!

To do

Miniprep 5 liquid cultures

pchBA PCR

1. Lets try to amplify out pchBA together and seperately
2. 3 test tubes + 1 control
3. Dilute new Invitrogen pchBA primers into working stocks
4. Tube 1 (pchBA):
5. Tube 1 (pchBA):
   • 49 μL PCR supermix
   • 1 μL pME3366 template
   • .6 μL pchB Forward
   • .6 μL pchA Reverse
6. Tube 2 (pchB):
   • 49 μL PCR supermix
   • 1 μL pME3366 template
   • .6 μL pchB Forward
   • .6 μL pchB Reverse
7. Tube 3 (pchA):
   • 49 μL PCR supermix
   • 1 μL pME3366 template
   • .6 μL pchA Forward
   • .6 μL pchA Reverse