IGEM:MIT/2006/Notebook/2006-7-31: Difference between revisions
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==Ligations== | ==Ligations== | ||
#R0040+1->Prom-RBS-BSMT-Term | #R0040+1->Prom-RBS-BSMT-Term | ||
#2+Term->RBS-ATF1<sub>mut</sub>-Term | #2+Term->RBS-ATF1<sub>mut</sub>-Term | ||
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#R0040+E0840 CP-RBS-GFP-Term | #R0040+E0840 CP-RBS-GFP-Term | ||
#osmY+RBS-SAMT-Term | #osmY+RBS-SAMT-Term | ||
==pchBA PCR== | ==pchBA PCR== | ||
Revision as of 08:41, 31 July 2006
to do
- find a new glycerol storage box and transfer 7/29's new glycerols into it
- nanodrop 23 minipreps from 7/29 and prepare 23 small tubes for sequencing order
- digestions+ligations+transformations (design a plan)
- troubleshoot pchBA (w/ mutagenesis ??) PCR
- fax offices about p. shuttle vectors
- link our system device diagrams on main wiki page
Current products
- RBS-CDSBSMT-Term
- RBS-ATF1mut
- SPP-RBS-GFP-Term
- ATF1mut-Term
Ligations
- R0040+1->Prom-RBS-BSMT-Term
- 2+Term->RBS-ATF1mut-Term
- RBS+4->RBS-ATF1mut-Term
- R0040+E0840 CP-RBS-GFP-Term
- osmY+RBS-SAMT-Term
pchBA PCR
Ran pchBA PCR with gradient
Sequencing Results
Looked at R40 and B30/32 sequencing results
