IGEM:MIT/2006/Notebook/2006-7-31: Difference between revisions
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==to do== | ==to do== | ||
#find a new glycerol storage box and transfer 7/29's new glycerols into it | #find a new glycerol storage box and transfer 7/29's new glycerols into it '''-done''' | ||
#nanodrop 23 minipreps from 7/29 and prepare | #nanodrop 23 minipreps from 7/29 and prepare 46 small tubes for sequencing order '''-done''' | ||
#digestions+ligations+transformations (design a plan) | #digestions+ligations+transformations (design a plan) | ||
#troubleshoot pchBA (w/ | #troubleshoot pchBA (w/out silent mutation) PCR | ||
#fax offices about p. shuttle vectors | #fax offices about p. shuttle vectors | ||
#link our system device diagrams on main wiki page | #link our system device diagrams on main wiki page | ||
Revision as of 11:38, 31 July 2006
to do
- find a new glycerol storage box and transfer 7/29's new glycerols into it -done
- nanodrop 23 minipreps from 7/29 and prepare 46 small tubes for sequencing order -done
- digestions+ligations+transformations (design a plan)
- troubleshoot pchBA (w/out silent mutation) PCR
- fax offices about p. shuttle vectors
- link our system device diagrams on main wiki page
Current products
- RBS-CDSBSMT-Term
- RBS-ATF1mut
- SPP-RBS-GFP-Term
- ATF1mut-Term
Digestions
- RBS30, 32-CDS-Term: XP
- RBS30, 32-ATF1: ES
- osmY: SP
- ATF1+Term: XP
- R0040: SP
- E0840: XP
Digests needed for ligation in our possession
- B0015: EX
- B0030: SP
- B0032: SP
Ligations
- R0040+1->Prom-RBS-BSMT-Term
- 2+Term->RBS-ATF1mut-Term
- RBS+4->RBS-ATF1mut-Term
- R0040+E0840 CP-RBS-GFP-Term
- osmY+RBS-SAMT-Term
pchBA PCR
Ran pchBA PCR with gradient
Sequencing Results
Looked at R40 + B30/32 sequencing results. Seems that the promoter is missing and we will be going with different set of ligations.
