IGEM:MIT/2006/Notebook/2006-7-6: Difference between revisions
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Revision as of 07:06, 6 July 2006
Number of Transformants from 7/5 SAMT-, BAMT-, & ATF1-BB Ligations/Transformations
| pUC19-Fresh Top 10 | E0040BB (+Ctrl) | BSMT-BB (+ Ctrl) | BAMT-BB | SAMT-BB | ATF1-BB | |
|---|---|---|---|---|---|---|
| 70 | lots (~300) | 11 | 1 | 14 | 1 |
We can make a few conclusions from these data:
- The old Top 10 cells are probably not suitable for use (despite colonies appearing at the 200μ plating volume).
- Barry's E0040 positive control worked.
- Our ligation was "succcessful", as colonies did appear on our plates.
