IGEM:MIT/2006/Notebook/2006-8-1: Difference between revisions

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==Run Gel of BAT2 and THI3 PCR Products==
==Run Gel of BAT2 and THI3 PCR Products==
Nothing showed- rePCR
Nothing showed- PCR BAT2 and THI3 again


==PCR again BAT2 and TH
==Fax Dr. Schweizer for pUCP18 etc.==
==Fax Dr. Schweizer for pUCP18 etc.==
Fax number is most likely dead or old  
Fax number is most likely dead or old  

Revision as of 11:10, 1 August 2006

to do (am)

  1. START ASAP!!!: repeat failed 7/31 digestions
  2. order new pchA reverse primer (ask Tom for help?)
  3. test osmY+E0840(A,B,C) part with plate reader

to do (pm)

  1. nanodrop and run a gel of digest products (NO GEL EXTRACTION)
  2. do ligations using new 8/1 digest products
  3. transform into new top10 cells

Miniprepped LCs

30-ATF1 ABC and R0040

Run Gel of BAT2 and THI3 PCR Products

Nothing showed- PCR BAT2 and THI3 again

Fax Dr. Schweizer for pUCP18 etc.

Fax number is most likely dead or old

Repeat Digestions (same as 7/31)

  1. 30B 32A 32C: RCT
  2. 32C 30A 30B: 30C RA
  3. B C: AT
  4. R0040

Reminder: Cut Pieces We Have

  1. B0015: EX
  2. B0030: SP
  3. B0032: SP
  4. R0040:SP (didn't show with 5μL loaded on wide lane gel, but could still be there)
  5. More on [[../../Index | topical index]]

ligations

transformations (@ 2:30 pm)

  1. prepare 9 plates, review the resistance of backbones
  2. use fresh Top10 cells, pUC19 control, 200 μL
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