IGEM:MIT/2006/Notebook/2006-8-1: Difference between revisions
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==Run Gel of BAT2 and THI3 PCR Products== | ==Run Gel of BAT2 and THI3 PCR Products== | ||
Nothing showed- rePCR | |||
==PCR again BAT2 and TH | |||
==Fax Dr. Schweizer for pUCP18 etc.== | ==Fax Dr. Schweizer for pUCP18 etc.== | ||
Fax number is most likely dead or old | Fax number is most likely dead or old |
Revision as of 11:09, 1 August 2006
to do (am)
- START ASAP!!!: repeat failed 7/31 digestions
- order new pchA reverse primer (ask Tom for help?)
- test osmY+E0840(A,B,C) part with plate reader
to do (pm)
- nanodrop and run a gel of digest products (NO GEL EXTRACTION)
- do ligations using new 8/1 digest products
- transform into new top10 cells
Miniprepped LCs
30-ATF1 ABC and R0040
Run Gel of BAT2 and THI3 PCR Products
Nothing showed- rePCR
==PCR again BAT2 and TH
Fax Dr. Schweizer for pUCP18 etc.
Fax number is most likely dead or old
Repeat Digestions (same as 7/31)
- 30B 32A 32C: RCT
- 32C 30A 30B: 30C RA
- B C: AT
- R0040
Reminder: Cut Pieces We Have
- B0015: EX
- B0030: SP
- B0032: SP
- R0040:SP (didn't show with 5μL loaded on wide lane gel, but could still be there)
- More on [[../../Index | topical index]]
ligations
transformations (@ 2:30 pm)
- prepare 9 plates, review the resistance of backbones
- use fresh Top10 cells, pUC19 control, 200 μL