IGEM:MIT/2006/Notebook/2006-8-1

to do (am)

1. miniprep/nanodrop 7 overnight liquid cultures
2. START ASAP!!!: repeat failed 7/31 digestions
3. ask Isadora to help with fax machine
4. order new pchA reverse primer (ask Tom for help?)
5. run a gel of BAT2 and THI3 pcr products
6. test osmY+E0840(A,B,C) part with plate reader

to do (pm)

1. nanodrop and run a gel of digest products (NO GEL EXTRACTION)
2. do ligations using new 8/1 digest products
3. transform into new top10 cells

repeat failed digestions

(same as 7/31)

reminder: cut pieces we have

1. B0015: EX
2. B0030: SP
3. B0032: SP
4. R0040:SP (didn't show with 5μL loaded on wide lane gel, but could still be there)

transformations (@ 2:30 pm)

1. prepare 9 plates, review the resistance of backbones
2. use fresh Top10 cells, pUC19 control, 200 μL