IGEM:MIT/2006/Notebook/2006-8-10

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(to do)
(to do)
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#plate reader test
#plate reader test
#PCR pchBA with 5% Betane
#PCR pchBA with 5% Betane
 +
#site-directed mutagenesis on banana generating device (with both RBS30 and RBS32), THI3 in TOPO vector with NEW mut primers
 +
#clone WGD into pseudomonas shuttle vector

Revision as of 17:54, 9 August 2006

to do

  1. GC work with new smell samples
  2. Miniprep all LCs/glycerols
    • Make 10 biobrick named glycerols and send for sequencing
  3. Make LCs of IK transformants
  4. plate reader test
  5. PCR pchBA with 5% Betane
  6. site-directed mutagenesis on banana generating device (with both RBS30 and RBS32), THI3 in TOPO vector with NEW mut primers
  7. clone WGD into pseudomonas shuttle vector
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