IGEM:MIT/2006/Notebook/2006-8-10: Difference between revisions
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#site-directed mutagenesis on banana generating device (with both RBS30 and RBS32), THI3 in TOPO vector with NEW mut primers | #site-directed mutagenesis on banana generating device (with both RBS30 and RBS32), THI3 in TOPO vector with NEW mut primers | ||
#clone WGD into pseudomonas shuttle vector | #clone WGD into pseudomonas shuttle vector | ||
==Indole KO Strain== | |||
Revision as of 07:29, 10 August 2006
to do
- GC work with new smell samples
- Miniprep all LCs/glycerols
- Make 10 biobrick named glycerols and send for sequencing
- Make LCs of IK transformants
- plate reader test
- PCR pchBA with 5% Betane
- site-directed mutagenesis on banana generating device (with both RBS30 and RBS32), THI3 in TOPO vector with NEW mut primers
- clone WGD into pseudomonas shuttle vector
