IGEM:MIT/2006/Notebook/2006-8-11: Difference between revisions

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|''IA-OH greatly overpowering''
|''IA-OH greatly overpowering''
|-
|-
|'''150 min'''
|'''120 min'''
|''slight hint of mint; normal IKO-smell prevalent''
|'''noticeable mint smell; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''strong banana scent'''
|''banana smell is faint; IA-OH smell prevalent''
|-
|-
|'''210 min'''
|'''180 min'''
|}
|}

Revision as of 09:41, 11 August 2006

To do

  1. smell overnight IK smell testers and after making glycerols from regular batch then add precursor to the daytime smell testers -done
  2. analyze sequencing from weds [full assemblies]
  3. List Biobrick Molecules
    • Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, BO could you put in links to each part's registry page on the index!)
  4. digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest
  5. ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm
  6. autoclave waste
  7. digest pCHBA with E and S and backbone with E and S and ligate
  8. mutagenesis of BAT2, transformation of BAT2, ATF1, and THI3 mutagenesis
  9. LCs of R0040.E0840 and osmY.E0840s
  10. GC of smell tests
  11. miniprep RBS.coding.terminators to inverters

Preliminary Smell Test: Indole KO Strain (André)

Experiment description

  • We would like to know the amount of time it takes to produce the final volatile compound.
    • I added SA (2mM) and IA-OH (10mM) to 5mL pre-grown Indole KO cultures.
    • Cultures containing J45100 and J45220 were further incubated at room temperature (25C)
    • Cultures containing J45120, J45170, and J45200 were further incubated (with vigorous shaking) at 37C
J45100 J45120 J45170 J45200 J45220
15 min smells pretty bad, i.e. like regular Indole KO cells smells pretty bad, i.e. like regular Indole KO cells smells pretty bad, i.e. like regular Indole KO cells faint smell of banana; smell of IA-OH lingering IA-OH greatly overpowering
30 min slight hint of mint; normal IKO-smell prevalent very slight hint of mint; normal IKO-smell prevalent smells pretty bad, i.e. like regular Indole KO cells noticeable smell of banana, stronger than at 15 min IA-OH greatly overpowering
60 min slight hint of mint; normal IKO-smell prevalent noticeable mint smell; normal IKO-smell prevalent smells pretty bad, i.e. like regular Indole KO cells powerful banana scent IA-OH greatly overpowering
90 min slight hint of mint; normal IKO-smell prevalent noticeable mint smell; normal IKO-smell prevalent smells pretty bad, i.e. like regular Indole KO cells strong banana scent IA-OH greatly overpowering
120 min slight hint of mint; normal IKO-smell prevalent noticeable mint smell; normal IKO-smell prevalent smells pretty bad, i.e. like regular Indole KO cells strong banana scent banana smell is faint; IA-OH smell prevalent
180 min
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