IGEM:MIT/2006/Notebook/2006-8-11: Difference between revisions
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==To do== | ==To do== | ||
#smell overnight IK smell testers and '''after making glycerols from regular batch''' then add precursor to the daytime smell testers '''-done''' | #smell overnight IK smell testers and '''after making glycerols from regular batch''' then add precursor to the daytime smell testers '''-done''' | ||
#analyze sequencing from weds [full assemblies] | #analyze sequencing from weds [full assemblies] '''veena, afternoon''' | ||
#List Biobrick Molecules | #List Biobrick Molecules | ||
#*Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, '''BO''' could you put in links to each part's registry page on the index!) | #*Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, '''BO''' could you put in links to each part's registry page on the index!) | ||
#digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest | #digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest '''digest started 12:30''' | ||
#ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm | #ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm | ||
#autoclave waste | #autoclave waste | ||
#digest pCHBA with E and S and backbone with E and S and ligate | #digest pCHBA with E and S and backbone with E and S and ligate '''digest started 12:30''' | ||
#mutagenesis of BAT2, transformation of BAT2, ATF1, and THI3 mutagenesis | #mutagenesis of BAT2, transformation of BAT2, ATF1, and THI3 mutagenesis '''Bo and Stephen pm''' | ||
#LCs of R0040.E0840 and osmY.E0840s | #LCs of R0040.E0840 and osmY.E0840s | ||
#GC of smell tests | #GC of smell tests '''decided to run it when we have more controlled samples to run''' | ||
#miniprep RBS.coding.terminators to inverters | #miniprep RBS.coding.terminators to inverters '''digests of these started at 12:30''' | ||
==''Preliminary'' Smell Test: Indole KO Strain ([[User:Dagreen|André]])== | ==''Preliminary'' Smell Test: Indole KO Strain ([[User:Dagreen|André]])== | ||
Revision as of 09:53, 11 August 2006
To do
- smell overnight IK smell testers and after making glycerols from regular batch then add precursor to the daytime smell testers -done
- analyze sequencing from weds [full assemblies] veena, afternoon
- List Biobrick Molecules
- Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, BO could you put in links to each part's registry page on the index!)
- digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest digest started 12:30
- ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm
- autoclave waste
- digest pCHBA with E and S and backbone with E and S and ligate digest started 12:30
- mutagenesis of BAT2, transformation of BAT2, ATF1, and THI3 mutagenesis Bo and Stephen pm
- LCs of R0040.E0840 and osmY.E0840s
- GC of smell tests decided to run it when we have more controlled samples to run
- miniprep RBS.coding.terminators to inverters digests of these started at 12:30
Preliminary Smell Test: Indole KO Strain (André)
Experiment description
- We would like to know the amount of time it takes to produce the final volatile compound.
- I added SA (2mM) and IA-OH (10mM) to 5mL pre-grown Indole KO cultures.
- Cultures containing J45100 and J45220 were further incubated at room temperature (25C)
- Cultures containing J45120, J45170, and J45200 were further incubated (with vigorous shaking) at 37C
| J45100 | J45120 | J45170 | J45200 | J45220 | |
|---|---|---|---|---|---|
| 15 min | smells pretty bad, i.e. like regular Indole KO cells | smells pretty bad, i.e. like regular Indole KO cells | smells pretty bad, i.e. like regular Indole KO cells | faint smell of banana; smell of IA-OH lingering | IA-OH greatly overpowering |
| 30 min | slight hint of mint; normal IKO-smell prevalent | very slight hint of mint; normal IKO-smell prevalent | smells pretty bad, i.e. like regular Indole KO cells | noticeable smell of banana, stronger than at 15 min | IA-OH greatly overpowering |
| 60 min | slight hint of mint; normal IKO-smell prevalent | noticeable mint smell; normal IKO-smell prevalent | smells pretty bad, i.e. like regular Indole KO cells | powerful banana scent | IA-OH greatly overpowering |
| 90 min | slight hint of mint; normal IKO-smell prevalent | noticeable mint smell; normal IKO-smell prevalent | smells pretty bad, i.e. like regular Indole KO cells | strong banana scent | IA-OH greatly overpowering |
| 120 min | slight hint of mint; normal IKO-smell prevalent | noticeable mint smell; normal IKO-smell prevalent | smells pretty bad, i.e. like regular Indole KO cells | strong banana scent | banana smell is faint; IA-OH smell prevalent |
| 180 min |
