IGEM:MIT/2006/Notebook/2006-8-11: Difference between revisions
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==To do== | ==To do== | ||
#smell overnight IK smell testers and '''after making glycerols from regular batch''' then add precursor to the daytime smell testers | |||
#BAT2 mut primers will arrive...do site directed mutagenesis on BAT2 and pchBA | |||
#analyze sequencing from weds [full assemblies] | |||
#List Biobrick Molecules | |||
#*Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, '''BO''' could you put in links to each part's registry page on the index!) | |||
#digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest | |||
#ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm |
Revision as of 14:33, 10 August 2006
To do
- smell overnight IK smell testers and after making glycerols from regular batch then add precursor to the daytime smell testers
- BAT2 mut primers will arrive...do site directed mutagenesis on BAT2 and pchBA
- analyze sequencing from weds [full assemblies]
- List Biobrick Molecules
- Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, BO could you put in links to each part's registry page on the index!)
- digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest
- ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm