IGEM:MIT/2006/Notebook/2006-8-11
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To do
- smell overnight IK smell testers and after making glycerols from regular batch then add precursor to the daytime smell testers
- analyze sequencing from weds [full assemblies]
- List Biobrick Molecules
- Lets update our index with our numbering system and all the biobrick constructs we believe that we have (and then we can check them off as we get the sequence results back. Also, BO could you put in links to each part's registry page on the index!)
- digest WGD with EP and run on a gel with 8/10 pUCP22:EP digest
- ligate WGD into EP cut pUCP22 shuttle vector and transform into Top10 in the pm
- autoclave waste
- digest pCHBA with E and S and backbone with E and S and ligate
- mutagenesis of BAT2, transformation of BAT2, ATF1, and THI3 mutagenesis
- LCs of R0040.E0840 and osmY.E0840s
- GC of smell tests
- miniprep RBS.coding.terminators to inverters
Preliminary Smell Test: Indole KO Strain (AG)
Experiment description
- We would like to know the amount of time it takes to produce the final volatile compound.
- I added SA (2mM) and IA-OH (10mM) to 5mL pre-grown Indole KO cultures.
- Cultures containing J45100 and J45220 were incubated at room temperature (25C)
- Cultures containing J45120, J45170, and J45200 were incubated (with vigorous shaking) at 37C
J45100 | J45120 | J45170 | J45200 | J45220 | |
---|---|---|---|---|---|
15 min | smells pretty bad, i.e. like regular Indole KO cells | smells pretty bad, i.e. like regular Indole KO cells | smells pretty bad, i.e. like regular Indole KO cells | faint smell of banana; smell of IA-OH lingering | IA-OH greatly overpowering |
30 min | slight hint of mint; normal IKO-smell prevalent | very slight hint of mint; normal IKO-smell prevalent | smells pretty bad, i.e. like regular Indole KO cells | noticeable smell of banana, stronger than at 15 min | IA-OH greatly overpowering |
60 min | |||||
90 min | |||||
120 min | |||||
150 min |