IGEM:MIT/2006/Notebook/2006-8-14: Difference between revisions

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We hope to generate data that resembles the following:
We hope to generate data that resembles the following:
{| border="1" cellpadding="5"
!|
!|''J45100''
!|J45120
!|J45170
!|J45200
!|''J45220''
|-
|'''15 min'''
|''smells pretty bad, i.e. like regular Indole KO cells''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''faint smell of banana; smell of IA-OH lingering'''
|''IA-OH greatly overpowering''
|-
|'''30 min'''
|''slight hint of mint; normal IKO-smell prevalent''
|'''very slight hint of mint; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''noticeable smell of banana, stronger than at 15 min'''
|''IA-OH greatly overpowering''
|-
|'''60 min'''
|''slight hint of mint; normal IKO-smell prevalent''
|'''noticeable mint smell; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''powerful banana scent'''
|''IA-OH greatly overpowering''
|-
|'''90 min'''
|''slight hint of mint; normal IKO-smell prevalent''
|'''noticeable mint smell; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''strong banana scent'''
|''IA-OH greatly overpowering''
|-
|'''120 min'''
|''slight hint of mint; normal IKO-smell prevalent''
|'''noticeable mint smell; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''strong banana scent'''
|''banana smell is faint; IA-OH smell prevalent''
|-
|'''240 min'''
|''very slight hint of mint; normal IKO-smell prevalent''
|'''faint mint smell; normal IKO-smell prevalent'''
|'''smells pretty bad, i.e. like regular Indole KO cells'''
|'''weakened banana scent'''
|''IA-OH smell prevalent''
|}


====Conclusions====
====Conclusions====

Revision as of 12:40, 11 August 2006

Preliminary Smell Test II (André)

Experiment description

  • We would like to know the amount of cells (and, therefore, roughly the amount of protein) necessary to produce the smells

Proposed Experimental Design

  • Add appropriate amounts of precursors to media with roughly equivalent numbers of cells
  • Smell cultures at different time intervals
  • Read OD at different time intervals.

We hope to generate data that resembles the following:

Conclusions

  • osmY is probably not correctly assembled in the BioBrick (J45140).
  • Some smell is obtained from cells that are incubated at 25C, indicating that ATF1 protein is present in a sufficient amount after overnight culturing.
  • Smell is obtained much more quickly when incubation is resumed at 37C as opposed to 25C. This is likely because the ATF1 protein present in the overnight culture is supplemented by increased protein production at 37C.
  • Because smell (banana) was detected after only 15 min of incubation at 37C (J45200), this means that sufficient IA-OH enters the cells within 15 minutes of addition.
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