IGEM:MIT/2006/Notebook/2006-8-16: Difference between revisions

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==to do==
==to do==
#miniprep all LCs and sequence
#miniprep all LCs and sequence '''-done'''
#check incoming sequence results (and judge semi-solid parts in freezer)
#check incoming sequence results (and judge semi-solid parts in freezer)
#site-directed mutagenesis for pchBA-B0015 and correct TH13
#site-directed mutagenesis for pchBA-B0015 and correct TH13

Revision as of 09:55, 16 August 2006

to do

  1. miniprep all LCs and sequence -done
  2. check incoming sequence results (and judge semi-solid parts in freezer)
  3. site-directed mutagenesis for pchBA-B0015 and correct TH13
  4. digest osmY (long new, short new, short old) with ES and 3-part assemble into biobrick format (plus with E0840)
  5. get new registry uncut backbones (A/K and A/T, etc.) and cut with EP
  6. acknowledgement link, and iGEM main page summary/picture
  7. team meet about you know what
  8. ligations/transformations
  9. ppm
  10. glycerol updating