IGEM:MIT/2006/Notebook/2006-8-16

to do

1. miniprep all LCs and sequence
2. check incoming sequence results (and judge semi-solid parts in freezer)
3. site-directed mutagenesis for pchBA-B0015 and correct TH13
4. digest 30.J45008.30 with XP and connect it to R0040
5. digest osmY (long new, short new, short old) with ES and 3-part assemble into biobrick format (plus with E0840)
6. get new registry uncut backbones (A/K and A/T, etc.) and cut with EP
7. acknowledgement link, and iGEM main page summary/picture
8. team meet about you know what
9. ligations/transformations