IGEM:MIT/2006/Notebook/2006-8-19

SAGD stuff

1. 16 minipreps of pchBA-B0015 muts
2. digestions:
   • pchBA-B0015 muts with: XP (RUN A GEL!! b/c pchA has a PstI site)
3. ligations: all good
4. transformations: on A/C plates

IAGD stuff

1. digestions:
   • good 30-Bat2-30 with: ES
   • THI3 with: XP

shifts

1. Kate: minipreps of pchBA and LC/glycerol of Q04400 and pUCP22
2. Stephen: plate reader and osmY analysis (+ sunday)
3. Andre: Glycerols of IK cells without substrate, smelling of IK cells with substrate, set-up new experiments (??)
4. Bo: 18 digests, pour and load 2 gels
5. Veena: read gel, design good ligations (remember to connect both RBS), ligate, transformation