IGEM:MIT/2006/Notebook/2006-8-2: Difference between revisions
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==to do== | ==to do== | ||
# | #analyze 42 sequence results using prepared contigs in VectorNTI -'''done. unfortunately our analysis shows that nothing seems to have inserted. Perhaps digests are not being run long enough? also, pchBA mutagenesis was unsuccessful. | ||
#start isoamyl alcohol generating device work | #start isoamyl alcohol generating device work | ||
#* | #*PCR BAT2 and THI3 again to get higher DNA concentrations for digesting/ligating on 8/3 | ||
#team meeting to discuss topics for meeting | |||
#(3 pm) meeting with advisors | #(3 pm) meeting with advisors | ||
# | #run gel of PCR products/PCR cleanup and nanodrop | ||
#make liquid cultures A,B,C from each potentially useful transformant plate: | |||
==meeting topics== | ==meeting topics== | ||
Revision as of 10:06, 2 August 2006
to do
- analyze 42 sequence results using prepared contigs in VectorNTI -done. unfortunately our analysis shows that nothing seems to have inserted. Perhaps digests are not being run long enough? also, pchBA mutagenesis was unsuccessful.
- start isoamyl alcohol generating device work
- PCR BAT2 and THI3 again to get higher DNA concentrations for digesting/ligating on 8/3
- team meeting to discuss topics for meeting
- (3 pm) meeting with advisors
- run gel of PCR products/PCR cleanup and nanodrop
- make liquid cultures A,B,C from each potentially useful transformant plate:
meeting topics
- troubleshoot pchBA primers
- troubleshoot pchBA mutagenesis
liquid cultures from potentially useful plates
- 7/31 plates: 4, 5, 6, 7, 8
- 8/1 plates: 7, 8, 9
