IGEM:MIT/2006/Notebook/2006-8-2

to do

1. analyze 42 sequence results using prepared contigs in VectorNTI -done. unfortunately our analysis shows that nothing seems to have inserted. Perhaps digests are not being run long enough? also, pchBA mutagenesis was unsuccessful.
2. start isoamyl alcohol generating device work
   • PCR BAT2 and THI3 again to get higher DNA concentrations for digesting/ligating on 8/3
3. (3 pm) meeting with advisors
4. run gel of PCR products/PCR cleanup and nanodrop BAT2 and THI3
5. colony PCRs
6. make liquid cultures A,B,C from each potentially useful transformant plate:

meeting topics

1. troubleshoot pchBA primers
2. troubleshoot pchBA mutagenesis
3. troubleshoot digests/failed assemblies
4. timeline of assembly work (best case scenario)

liquid cultures from potentially useful plates

1. 7/31 plates: 4, 5, 6, 7, 8
   • 4/5/6/7 = maybe ATF1mut-Term (colony PCR to check first)
   • 8 = ROO40-E0840
2. 8/1 plates: 7, 8, 9
   • 7/8/9 = maybe ATF1mut-Term (colony PCR to check first)