IGEM:MIT/2006/Notebook/2006-8-21: Difference between revisions
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==Ligations== | ==Ligations== | ||
Ligate osmY<sub>ES</sub> with XP cut: | |||
#Q99ABC | #Q99ABC | ||
| Line 7: | Line 7: | ||
#Q219ABC | #Q219ABC | ||
#QE0840ABC | #QE0840ABC | ||
('''after''' running a gel to make sure inverter Q044000 is around) | |||
==Mutagenesis== | ==Mutagenesis== | ||
Revision as of 09:27, 21 August 2006
Ligations
Ligate osmYES with XP cut:
- Q99ABC
- Q119ABC
- Q199ABC
- Q219ABC
- QE0840ABC
(after running a gel to make sure inverter Q044000 is around)
Mutagenesis
Did mutagenesis on 30-BAT2-30-THI3 to get rid of site in THI3 and on pchBA-15
Transformations
Transformed ligations and mutagenesis products
