IGEM:MIT/2006/Notebook/2006-8-25
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Things done today:
- Minipreped, glyceroled, digested, ran on a gel, ligated to R0011 in 1AT3, and transformed rbs.pchba.term structures
- Performed site-directed mutagenesis on the correct minipreps of the 30.bat2.30.thi3 structures
- Glyceroled the correct minipreps of the 30.bat2.30.thi3 structures
- Sequenced rbs.pchba.term structures along with the osmy.inverter.xxx structures
- Examined some of the sequencing results although many were inconclusive due to bad sequencing
- Threw away the indole-knockout osmy.inverter.xxx structures since we found that the supposed cells were nasty smelling
Note: In the future, for competent indole knockout cells, use the ones labeled "IKN" in the indole-knockout box.